Arsénio M. Fialho

POSITION: Associate Professor with Habilitation

Leader of the research theme: Bacterial pathogenesis and therapeutic products

E-mail: afialho@tecnico.ulisboa.pt

Research Topics

  • Identification and characterization of trimeric autotransporter adhesins (TAA) in Bcc bacteria: mechanisms of action and their role in virulence
  • Microbial-based therapy of cancer: bacterial protein azurin and derived peptides as novel anticancer agents
  • Targeted p28-nanoparticles for cancer therapy

Research Team

Nuno Bernardes

Dalila Mil-Homens

Ana Rita Garizo

Andreia Pimenta

Education

  • Graduation in Biology, Faculty of Science, University of Lisbon, Lisbon, Portugal, 1986
  • Visiting graduate student, Dept. Microbiology and Immunology, University of Illinois at Chicago, USA, Chicago, 1988/89
  • Ph.D in Biotechnology, Instituto Superior Técnico, 1996
  • Habilitation in Biotechnology, Instituto Superior Técnico, 2011
  • Sabbatical leave:
    • Atelier de BioInformatique, University Paris VI, Paris, France, 2004
    • Departments of Microbiology and Immunology and Surgical Oncology, University of Illinois at Chicago, Chicago, USA, 2005

Positions

  • Teaching Assistant, IST, 1987 – 1996
  • Assistant Professor, IST, 1996 – 2004
  • Visiting Research Associate Professor, Department of Surgical Oncology, University of Illinois at Chicago, June-August 2005
  • Associate Professor, IST, 2004 – 2011
  • Associate Professor with Habilitation, IST, 2011 – present 

Selected Publications

Bernardes N, Garizo AR, Pinto SN, Caniço B, Perdigão C, Fernandes F, Fialho AM. “Azurin interaction with the lipid raft components ganglioside GM-1 and caveolin-1 increases membrane fluidity and sensitivity to anti-cancer drugs”. Cell Cycle,2018,doi: 10.1080/15384101.2018.1489178.

Mil-Homens D., Pinto S.N., Matos R.G., Arraiano, C., Fialho A.M. “Burkholderia cenocepacia K56-2 trimeric autotransporter adhesin BcaA binds TNFR1 and contributes to induce airway inflammation” Cellular Microbiology, 19(4), 2017

N Bernardes, S Abreu, FA Carvalho, F Fernandes, NC Santos, AM Fialho “Modulation of membrane properties of lung cancer cells by azurin enhances the sensitivity to EGFR-targeted therapy and decreased β1 integrin-mediated adhesion” Cell Cycle, 15(11)1415-24 (2016) (DOI: 10.1080/15384101.2016.1172147, 2016

Mil-Homens D., Leça M.I., Fernandes F., Pinto S.N., Fialho A.M. “Characterization of BCAM0224, a multifunctional trimeric autotransporter from the human pathogen Burkholderia cenocepaciaJournal of Bacteriology  , 196(11):1968-79, 2014

Bernardes N., Ribeiro A. S., Abreu S., Vieira A.F., Carreto L., Santos M., Seruca R., Paredes J., Fialho A.M. “High-throughput molecular profiling of a P-cadherin overexpressing breast cancer model reveals new targets for the anti-cancer bacterial protein azurin” International Journal of Biochemistry & Cell Biology, 50:1-9, 2014

El-Kirat-Chatel S., Mil-Homens D., Beaussart A., Fialho A.M., Dufrêne Y.F. “Single-molecule atomic force microscopy unravels the binding mechanism of a Burkholderia cenocepacia trimeric autotransporter adhesin” Molecular Microbiology, 89(4):649-659, 2013

Bernardes N., Ribeiro A.S., Abreu S., Mota B., Matos R.G., Arraiano C.M., Seruca R., Paredes J., Fialho A.M. “The bacterial protein azurin impairs invasion and FAK/Src signaling in P-cadherin-overexpressing breast cancer cell models”  PLoS One, 8 (7), e69023, 2013

All Papers, Book Chapters and Patents

See all

Publications in peer reviewed journals:

Bernardes N, Garizo AR, Pinto SN, Caniço B, Perdigão C, Fernandes F, Fialho AM. “Azurin interaction with the lipid raft components ganglioside GM-1 and caveolin-1 increases membrane fluidity and sensitivity to anti-cancer drugs”. Cell Cycle,2018,doi: 10.1080/15384101.2018.1489178.

Mil-Homens D, Barahona S, Moreira RN, Silva IJ, Pinto SN, Fialho AM, Arraiano CM. “The stress-response protein BolA influences fitness and promotes Salmonella Typhimurium virulence.”Applied and Environmental Microbiology, 2018, doi: 10.1128/AEM.02850-17.

Romão D, Cavalheiro M, Mil-Homens D, Santos R, Pais P, Costa C, Takahashi-Nakaguchi A, Fialho AM, Chibana H, Teixeira MC “A new determinant of Candida glabrata virulence: the acetate exporter CgDtr1” Frontiers in Cellular and Infection Microbiology, Manuscript ID: 292948; 2017, doi: 10.3389/fcimb.2017.00473

Liliana O. Silva, Lígia S. Nobre, Dalila Mil-Homens, Arsénio Fialho, Lígia M. Saraiva. “Repair of Iron centers RIC protein contributes to the virulence of Staphylococcus aureus.“  Virulence, 2017, doi: 10.1080/21505594.2017.1389829

Mil-Homens D., Pinto S.N., Matos R.G., Arraiano, C., Fialho A.M. Burkholderia cenocepacia K56-2 trimeric autotransporter adhesin BcaA binds TNFR1 and contributes to induce airway inflammation” Cellular Microbiology, 19(4), 2017

Santos R, Costa C, Mil-Homens D, Romão D, de Carvalho CC, Pais P, Mira NP, Fialho AM, Teixeira MC. “The multidrug resistance transporters CgTpo1_1 and CgTpo1_2 play a role in virulence and biofilm formation in the human pathogen Candida glabrata.” Cellular Microbiology, 19(5), 2017

Moreira A.S., Mil-Homens D., Sousa S.A., Coutinho CP., Oliveira A.P., Ramos C.G., Santos S.S., Fialho A.M., Leitão J.H., and Sá-Correia I. “Variation of Burkholderia cenocepacia virulence potential during cystic fibrosis chronic lung infection” Virulence, 8(6): 782-796, 2017

Fialho AM, Bernardes N, Chakrabarty AM “Exploring the anticancer potential of the bacterial protein azurin" AIMS Microbiology 2(3):292-303, 2016

Bernardes N, Abreu S, Carvalho FA, Fernandes F, Santos NC, Fialho AM “Modulation of membrane properties of lung cancer cells by azurin enhances the sensitivity to EGFR-targeted therapy and decreased β1 integrin-mediated adhesion" Cell Cycle, 15(11):1415-24, 2016

Mil-Homens D, Ferreira-Dias S, Fialho AM, “Fish oils against Burkholderia and Pseudomonas aeruginosa: in vitro efficacy and their therapeutic and prophylactic effects on infected Galleria mellonella larvae", Journal of Applied Microbiology, 120(6):1509-19, 2016

Chakrabarty A.M., Bernardes N., Fialho A.M. “Bacterial Proteins and Peptides in Cancer Therapy: Today and Tomorrow” Bioengineered, 5:4 – 3, 2014

Mil-Homens D., Leça M.I., Fernandes F., Pinto S.N., Fialho A.M. “Characterization of BCAM0224, a multifunctional trimeric autotransporter from the human pathogen Burkholderia cenocepacia” Journal of Bacteriology, 196(11):1968-79, 2014  

Bernardes N., Ribeiro A. S., Abreu S., Vieira A.F., Carreto L., Santos M., Seruca R., Paredes J., Fialho A.M. “High-throughput molecular profiling of a P-cadherin overexpressing breast cancer model reveals new targets for the anti-cancer bacterial protein azurin” International Journal of Biochemistry & Cell Biology, 50:1-9, 2014  

Viegas S., Mil-Homens D., Fialho A.M., Arraiano C.M. “The virulence of Salmonella Typhimurium in the insect model Galleria mellonella is impaired by mutations in endoribonucleases E and III” Applied and Environmental Microbiology, 79(19):6124-33, 2013

El-Kirat-Chatel S., Mil-Homens D., Beaussart A., Fialho A.M., Dufrêne Y.F. “Single-molecule atomic force microscopy unravels the binding mechanism of a Burkholderia cenocepacia trimeric autotransporter adhesin” Molecular Microbiology, 89(4):649-659, 2013

Bernardes N., Ribeiro A.S., Abreu S., Mota B., Matos R.G., Arraiano C.M., Seruca R., Paredes J., Fialho A.M. “The bacterial protein azurin impairs invasion and FAK/Src signaling in P-cadherin-overexpressing breast cancer cell models” PLoS One, 8 (7), e69023, 2013

Bernardes N., Chakrabarty A.M., Fialho A.M. “Engineering of bacterial strains and their products for cancer therapy” Applied Microbiology and Biotechnology 97(12):5189-5199, 2013

Barbas A., Popescu A., Frazão C., Arraiano C.M., Fialho A.M. “Rossmann-fold motifs can confer multiple functions to metabolic enzymes: RNA binding and ribonuclease activity of a UDP-glucose dehydrogenase” Biochemical and Biophysical Research Communications 430: 218-224, 2013

Fialho A.M., Chakrabarty A.M. “Patent controversies and court cases: Cancer diagnosis, therapy and prevention” Cancer Biology and Therapy 13: 1229 – 1234, 2012

Mil-Homens D., Fialho A.M. “A BCAM0223 Mutant of Burkholderia cenocepacia is Deficient in Hemagglutination, Serum Resistance, Adhesion to Epithelial Cells and Virulence” PLoS One, 7(7):e41747, 2012

Avner B. S., Fialho A.M., Chakrabarty A.M. “Overcoming drug resistance in multi-drug resistant cancers and microorganisms: A conceptual framework” Bioengineered 3:262-270, 2012

Chelinho S., Moreira-Santos M., Silva C., Costa C., Viana P., Viegas A.C., Fialho A.M., Ribeiro R., Sousa J.P. “Semifield testing of a bioremediation tool for atrazine-contaminated soils: evaluating the efficacy on soil and aquatic compartments” Environmental Toxicology and Chemistry 31, 1564-72. doi: 10.1002/etc.1840, 2012

Matos R.G., Fialho A.M., Giloh M., Schuster G., Arraiano C.M.” The rnb Gene of Synechocystis PCC6803 Encodes a RNA Hydrolase Displaying RNase II and Not RNase R Enzymatic Properties” PLoS One 7(3):e32690, 2012

Mil-Homens D., Bernardes N., Fialho A.M. “The antibacterial properties of docosahexaenoic omega-3 fatty acid against the Cystic Fibrosis multi-resistant pathogen Burkholderia cenocepacia” FEMS Microbiology Letters 328(1):61-9, 2012. (abstract)

Mil-Homens D., Fialho A.M. “Trimeric autotransporter adhesins in members of the Burkholderia cepacia complex: a multifunctional family of proteins implicated in virulence” Frontiers in Cellular and Infection Microbiology 1:13, 2011 (abstract)

Fialho A.M., Salunkhe P., Manna S.K., Mahali S., Chakrabarty A.M. “Glioblastoma Multiforme: Novel Therapeutic Approaches” ISRN Neurology Article ID 642345, doi:10.5402/2012/642345, 2012 (abstract

Fialho A.M., Bernardes N., Chakrabarty A.M.”Recent patents on live bacteria and their products as potential anticancer agents” Recent Patents on Anti-Cancer Drug Discovery 7, 31-55, 2012 (abstract)

Fialho A.M., Chakrabarty A.M. “Law-medecine interfacing: patenting of human genes and mutations” Recent Patents on DNA & Gene Sequences 5, 81-85, 2011 (abstract)

Rocha J., Popescu A.O., Borges P., Mil-Homens D, Sá-Correia I., Fialho A.M., Frazão C. “Burkholderia cepacia UDP-glucose dehydrogenase (BceC) structure and the role of Tyr10 in the final hydrolysis of UGDs thioester intermediate” Journal of Bacteriology, 193, 3978-3987, 2011 (abstract)

Rocha J., Popescu A.O., Sá-Correia I., Fialho A.M., Frazão C. “Cloning, expression, crystallization and preliminary crystallographic studies of UgdG, and UDP-glucose dehydrogenase from Sphingomonas elodea ATCC 31461” Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 66 (Pt 1):69-72, 2010

Mil-Homens, E.P.C. Rocha,A.M. Fialho,  “Genome-wide analysis of DNA repeats of DNA repeats in Burkholderia cenocepacia J2315 identifies a novel adhesin-like gene unique to epidemic-associated strains of ET-12 lineage", Microbiology, 156, 1084-1096, 2010 (abstract)

Bernardes, R. Seruca, A.M. Chakrabarty,A.M. Fialho, “Microbial-based therapy of cancer: current progress and future prospects",Bioengineered Bugs 1(3), 178-190 , 2010 (abstract)

Rocha, A.T. Granja, I. Sá-Correia,A.M. Fialho, C. Frazão,  “Cloning, expression, crystallization and preliminary crystallographic studies of UgdG, and UDP-glucose dehydrogenase fromSphingomonas elodea ATCC 31461" Acta Crytallographica. Section F, Structural Biology and Crystallization Communications,  66, 269-271, 2010 (abstract)

Velho, C. Oliveira, J. Paredes, S. Sousa, M. Leite, P. Matos, F. Milanezi, A.S. Ribeiro, N. Mendes, D. Licastro, A. Karhu, M.J. Oliveira, M. Ligtenberg, R. Hamelin, F. Carneiro, A. Lindblom, P. Peltomaki, S. Castedo, Jr S. Schwartz, P. Jordan, L.A. Aaltonen, R.M.W. Hofstra, G. Suriano, E. Stupka,A.M. Fialho,  R. Seruca, 2009, “Mized lineage kinase 3 gene mutations in mismatch repair deficient gastrointestinal tumours",Human Molecular Genetics19(4), 697-706, 2010  (abstract)

Chelinho, M. Moreira-Santos, D. Lima, C. Silva, P. Viana, S. André, I. Lopes, R. Ribeiro,A.M. Fialho, C.A. Viegas, J.P. Sousa.2009. “Cleanup of atrazine contaminated soils: ecotoxicological study on the efficacy of a bioremediation tool with Pseudomonas sp. ADP". Journal of Soils and Sediments, 10, 568-578, 2010 (abstract)

Domingos, R.G. Matos, F.P. Reis,A.M. Fialho, A. Barbas, C.M. Arraiano, “Biochemical characterization of the RNaase II family of exoribonucleases from the human pathogensSalmonella typhimurium and Streptococcus pneumoniae“, Biochemistry 48(50):11848-11857, 2009 (abstract)

Kundu, J. Thomas,A.M. Fialho, J.M. Kwan, L. Moreira, M. Mahfouz, T.K. Das Gupta, A.M. Chakrabarty, “The anticancer activity of the  N-terminal CARD-like domain of Arginine Deiminase (ADI) from Pseudomonas aeruginosaLetters in Drug Design & Discovery, 6, 403-412, 2009

J.M. Kwan, A.M. Fialho, C.S. Hong, M. Kundu, T.K. Das Gupta, A.M. Chakrabarty, “Bacterial Proteins as Potential Drugs in the Treatment of Leukemia", Leukemia Research33, 1392-1399, 2009 (abstract)

V.M.F. Gonçalves, A. Reis, M.R.M. Domingues, J.A. Lopes-da-Silva, A.M. Fialho, L.M. Moreira, I. Sá-Correia, M.A. Coimbra (2008) “Structural analysis of gellans produced by Sphingomonas elodea strains by electrospray tandem mass spectrometry", Carbohydrate Polymers,  77, 10-19, 2009

M.T. Holden, H.M. Seth-Smith, L.C. Crossman, M. Sebaihia, S.D. Bentley, A.M. Cerdeño-Tárraga, N.R. Thomson, N. Bason, M.A. Quail, S. Sharp, I. Cherevach, C. Churcher, I. Goodhead, H. Hauser, N. Holroyd, K. Mungall, P. Scott, D. Walker, B. White, H. Rose, P. Iversen, D. Mil-Homens, E.P. Rocha, A.M. Fialho, A. Baldwin, C. Dowson, B.G. Barrell, J.R. Govan, P. Vandamme, C.A. Hart, E. Mahenthiralingam, J. Parkhill “The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients." Journal of Bacteriology191(1):261-277, 2009 (abstract)

Lima, P. Viana, S. André, S. Chelinho, C. Costa, R. Ribeiro, J.P. Sousa,A.M. Fialho, C.A. Viegas."Evaluating a bioremediation tool for atrazine contamination soils in open soil microcosmos: the effectiveness of bioaugmentation and biostimulation approaches". Chemosphere74, 187-192, 2009 (abstract)

A.M. Fialho, L.M. Moreira, A.T. Granja, A.O. Popescu, K. Hoffmann, I. Sá-Correia, “Occurrence, production and applications of gellan: current state and perspectives". Applied Microbiology and Biotechnology79, 889-900, 2008 (abstract)

Naguleswaran,A.M. Fialho, A. Chaudhari, C.S. Hong, A.M. Chakrabarty, W.J. Sullivan Jr., “Azurin-like protein blocks invasion ofToxoplasma gondii through potential interaction with parasite surface antigen SAG1" Antimicrobial Agents and Chemotherapy52, 402-408, 2008 (abstract)

A.M. Fialho, A.M. Chakrabarty,  “Recent Patents on Bacterial Proteins as Potential Anticancer Agents" Recent Patents on Anti-Cancer Drug Discovery,  3, 224-234, 2007

A.T. Granja, A. Popescu, A.R. Marques, I. Sá-Correia, A.M. Fialho, “Biochemical characterization and phylogenetic analysis of UDP-glucose dehydrogenase from the gellan gum producer Sphingomonas elodea ATCC  31461" Applied Microbiology and Biotechnology76, 1319-1327, 2007 (abstract)

Aragão,A.M. Fialho, A.R. Marques, E.P. Mitchell, I. Sá-Correia, C. Frazão, “The complex ofSphingomonas elodea ATCC 31461 Glucose-1-phosphate uridylyltransferase with glucose-1-phosphate reveals a novel quaternary structure, unique among Nucleoside Diphosphate-sugar pyrophosphorylase members", Journal of Bacteriology, 189, 4520–4528, 2007 (abstract)

A.M. Fialho, F.J. Stevens, T.K. das Gupta, A.M. Chakrabarty, “Beyond host-pathogen interactions: microbial defense strategy in the host environmental", Current Opinion in Biotechnology18, 279-286, 2007 (abstract)

Chaudhari, M. Mahfouz,A.M. Fialho, T. Yamada, A.T. Granja, Y. Zhu, W. Hashimoto, B. Schlarb-Ridley, W. Cho, T. Das Gupta, A.M. Chakrabarty, “Cupredoxin-Cancer Interrelationship:  Azurin Binding with EphB2,Interference of EphB2 Tyrosine Phosphorylation and Inhibition of Cancer Growth"Biochemistry46, 1799-1810, 2007 (abstract)

A.M. Fialho, T.K. das Gupta, A.M. Chakrabarty, “Designing promiscuous drugs? Look at what nature made!", Letters in Drug Design & Discovery4, 40-43, 2007 (abstract)

Aragão, A.R. Marques, C. Frazão, F.J. Enguita, M.A. Carrondo,A.M. Fialho, I. Sá-Correia, E.P. Mitchell, “Cloning, expression, purification, crystallization and preliminary structure determination of glucose-1-phosphate uridylytransferase (UgpG) fromSphingomonas elodea ATCC 31461 bound to glucose-1-phosphate", Acta Crystallographica, F62, 930-934, 2006 (abstract)

C.S. Hong, T. Yamada, W. Hashimoto, A.M. Fialho, T.K. das Gupta, A.M. Chakrabarty,"Disrupting the entry barrier and attacking brain tumors: The role of the Neisseria H.8 epitope and the Laz protein", Cell Cycle5:151633-16412006 (abstract)

Chaudhari,A.M. Fialho, D. Ratner, P. Gupta, C.S. Hong, S. Kahali, T. Yamada, K. Haldar, S. Murphy, W. Cho, V.S. Chauhan, T.K. das Gupta, A.M. Chakrabarty, “Azurin,Plasmodium falciparum and HIV/AIDS: Inhibition of parasitic and viral growth by azurin", Cell Cycle, 5:15, 1642-1648, 2006 (abstract)

Amblar, A. Barbas,A.M. Fialho, C.M. Arraiano, “Characterization of the functional domains ofEscherichia coli RNase II", Journal of Molecular Biology360, 921-933, 2006 (abstract)

Hiraoka, A.T.Granja,A.M. Fialho,  B. Schlarb-Ridley, T.K. Das Gupta, A.M. Chakrabarty, T. Yamada. “Human cytochrome c enters murine J7774 cells and causes G1 and G2/M cell cycle arrest and induction of apoptosis” Biochemical and Biophysical Research Communications338, 1284-1290, 2005 (abstract)

Yamada,A.M. Fialho, V. Punj, L. Bratescu, T. Das Gupta, A.M. Chakrabarty. “Internalization of bacterial redox protein azurin in mammalian cells: entry domain and specificity”Cellular Microbiology, 7, 1418-1431, 2005 (abstract)

Silva, A.R. Marques,A.M. Fialho, A.T. Granja, I. Sá-Correia, “Proteins Encoded bySphingomonas elodea ATCC 31461 rmlA and ugpG genes, involved in gellan gum biosynthesis, exhibit both dTDP- and UDP-Glucose pyrophosphorylase activities”,Applied and Environmental Microbiology71, 4703-4712, 2005  (abstract)

Silva,A.M. Fialho, I. Sá-Correia, R.G. Burns, L.J. Shaw, “Combined bioaugmentation and biostimulation to clean-up soil contaminated with high concentrations of atrazine",Environmental Science and Technology38, 632-637, 2004 (abstract)

A.R. Marques, P.M. Coutinho,  P.A. Videira, A.M. Fialho, I. Sá-Correia, “Sphingomonas paucimobilis b-glucosidase Bgl1: a member of a new bacterial subfamily in family 1 of glycoside hydrolases", Biochemical Journal370,793-804, 2003 (abstract)

A.R.Marques, P.B. Ferreira, I.Sá-Correia, A.M. Fialho, “Characterization of the ugpG gene encoding a UDP-glucose pyrophosphorylase from the gellan gum producer Sphingomonas paucimobilis ATCC 31461", Molecular Genetics and Genomics268, 816-824, 2003 (abstract)

Videira,A.M. Fialho, A.R.Marques, P.M. Coutinho, I. Sá-Correia, “Cloning and sequence analysis of the ces 10 gene encoding a Sphingomonas paucimobilis esterase", Applied Microbiology and Biotechnology61, 517-522, 2003 (abstract)

F.M.C. Santana, T. Pinto, A.M. Fialho, I. Sá-Correia, J. Empis , “Bacterial removal of quinolizidine alkaloids and other carbon sources from a Lupinus albus aqueous extract”, Journal of Agricultural and Food Chemistry50, 2318-2323, 2002 

Sá-Correia,A. M. Fialho, P. Videira , L. M. Moreira, A. R. Marques , Albano H., “Gellan gum biosynthesis inSphingomonas paucimobilis ATCC 31461: genes, enzymes and exopolysaccharide production  engineering", Journal of IndustrialMicrobiology and Biotechnology, 29, 170-176, 2002

P.A.Videira, A.M. Fialho, R.A. Geremia, C. Breton, I. Sá-Correia, “Biochemical characterisation of ß1-4 glucuronosyltransferase GelK in the gellan gum producing strain Sphingomonas paucimobilis ATCC31461", Biochemical Journal358, 457-464, 2001

Videira, L.L. Cortes,A.M. Fialhoand I. Sá-Correia, “Identification of the pgmG gene, encoding a bifunctional protein with phosphoglucomutase and phosphomannomutase activities, in the gellan gum producing strain Sphingomonas paucimobilisATCC 31461", Applied and Environmental Microbiology66, 2252-2258, 2000  (abstract)

I.M. Tavares, L. Jolly, F. Pompeo, J.H. Leitão, A.M. Fialho, I. Sá-Correia, D. Mengin-Lecreulx, “Identification of the Pseudomonas aeruginosa glmM gene encoding phosphoglucosamine mutase", Journal of Bacteriology182, 4453-4457, 2000  (abstract)

I.M. Tavares, J.H. Leitão, A.M. Fialho and I. Sá-Correia, “Pattern of changes in the activity of enzymes of GDP-D-mannuronic acid synthesis and in the level of transcription of algAalgC and algD genes accompanying the loss and emergence of mucoidy in Pseudomonas aeruginosa“, Research in Microbiology 150, 105-116, 1999 (abstract)

A.M. Fialho, L.O. Martins, M-L. Donval, J.H. Leitão, M.J. Ridout, A.J. Jay, V.J. Morris and I. Sá-Correia, “Structures and properties of gellan polymers produced by Sphingomonas paucimobilis ATCC 31461 from lactose compared with those produced from glucose and from cheese whey", Applied and Environmental Microbiology65, 2485-2491, 1999  (abstract) 

A.J. Jay, I.J. Colquhoun, M.J. Ridout, G.J. Brownsey, V.J. Morris, A.M. Fialho, J.H. Leitão and I. Sá-Correia, “Analysis of structure and function of gellans with different substitution patterns", Carbohydrate Polymers35, 179-188, 1998

J.A. Richau, D. Choquenet, A.M. Fialho, L.M. Moreira and I. Sá-Correia, “The Biosynthesis of the Exopolysaccharide Gellan Results in the Decrease of Sphingomonas paucimobilis Tolerance to Copper (Cu2+)", Enzyme and Microbial Technology20, 510-515, 1997. (abstract)

J.A. Richau, D. Choquenet,  A.M. Fialho, and I. Sá-Correia,  “Emergence of Cu++-tolerance mutants defective in gellan synthesis in Cu++-stressed cultures of Sphingomonas paucimobilis"Research in Microbiology148, 251-261, 1997 (abstract)

F.M.C. Santana, A.M. Fialho, I. Sá-Correia and J.M.A. Empis, “Isolation of bacterial strains capable of using lupanine, the predominant quinolizidine alkaloid in white lupin, as sole carbon and energy source", Journal of Industrial Microbiology , 17, 110-115, 1996.(abstract)

L.O. Martins, A.M. Fialho, P.L. Rodrigues,  and  I. Sá-Correia, “Gellan gum production and activity of biosynthetic enzymes in Sphingomonas paucimobilis colonial variants concerning mucoidy", Biotechnology and Applied Biochemistry24, 47-54, 1996

G.A. Monteiro, A.M. Fialho, S.J. Ripley, I. Sá-Correia, “Electrotransformation of gellan-gum producing and non-producing Pseudomonas elodea strains", Journal of Applied Bacteriology, 72, 423-428, 1992

J.H. Leitão, A.M. Fialho, I. Sá-Correia, “Effects of growth temperature on GDP-mannuronic acid-forming enzymes activities and alginate synthesis in variants of Pseudomonas aeruginosa“, Journal of General Microbiology138, 605-610, 1992

Shinabarger, A. Berry, T.B. May, R. Rothmel,A.M. Fialho, and  A.M. Chakrabarty, “Purification and characterization of phosphomannose isomerase-guanosine diphospho-D-mannose pyrophosphorylase-A bifunctional enzyme in the alginate biosynthesis pathway ofPseudomonas aeruginosa"Journal of Biological Chemistry266, 2080-2088, 1991

A.M. Fialho, G.A. Monteiro, and I. Sá-Correia, “Conjugal transfer of recombinant plasmids into gellan gum producing and non-producing variants of Pseudomonas elodea ATCC 31461", Letters in Applied Microbiology12, 85-87, 1991 (abstract)

A.M. Fialho, N.A. Zielinski, W.F. Fett, A.M. Chakrabarty  and A. Berry, “Distribution of alginate gene sequences in the Pseudomonas rRNA homology GroupI/Azomonas/Azotobacter lineage of Superfamily B Prokaryotes", Applied and Environmental Microbiology56, 436-443, 1990 (abstract)

H.L. Fernandes, M.M. Tomé, F.M. Lupi, A.M. Fialho, I. Sá-Correia, J.M. Novais, “Biosynthesis of high concentrations of an exopolysaccharide during the cultivation of the microalga Botryococcus braunii“, Biotechnology Letters11, 433-436, 1989

Berry, J. Devault, S. Roychoudhury, N. Zielinski, T.B. May, E.C. Wynne, R.K. Rothmel,A.M. Fialho, M. Mussein, V. Krylov, A.M. Chakrabarty, “Pseudomonas aeruginosainfection in Cystic Fibrosis: Molecular Genetic approaches to Medical problem", Chimica Oggi9, 13-19, 1988

Books edition:

A.M. Fialho, A.M. Chakrabarty, “Emerging Cancer Therapy: Microbial Approaches and Biotechnological tools", John Wiley & Sons, p. 432, 2010 (ISBN: 978-0-470-44467-2)

C.M. Arraiano, A.M. Fialho, “O Mundo do RNA: Novos Desafios e Perspectivas Futuras”, Lidel Edições Técnicas, Lisboa, Portugal, 2007

Book chapters:

A.M. Fialho, “Bactérias Inimigas de Tumores: Novas Terapias para Combater o Cancro” In: Laboratórios Abertos 2011” (M.A. Lemos, C. G. Azevedo eds) ISBN: 978-989-96933-2-6 (e-book; http://deq.ist.utl.pt/Publica/Laboratorios%20Abertos%202011.pdf), 2011

C.A. Viegas, S. Chelinho, M. Moreira-Santos, C. Costa, F.N. Gil, C. Silva, D. Lima, R. Ribeiro, J.P. Sousa, A.M. Fialho, “Bioremediation of soils contaminated with atrazine and other s/-triazine herbicides: current state and prospects", In: Advances in Environmental Research, Vol. 6 (Columbus F. ed), Nova Science Publishers, Inc. (NY, USA), 2010

A.M. Fialho, A.M. Chakrabarty, “Recent patents on bacterial proteins as potential anticancer agents", Chapter 27, p. 601-622, InFrontiers in Anti-Cancer Drug Discovery, Vol. 1 (M. Iqbal Choudhary, FRS Atta-ur-Rahman) Bentham Science Publishers, 2010

A.M. Fialho, A.M. Chakrabarty, “Promiscuous anticancer drugs from pathogenic bacteria: rational versus intelligent drug design", Chapter 8, p.181-198, InEmerging Cancer Therapy: Microbial Approaches and Biotechnological tools (A.M. Fialho, A.M. Chakrabarty, Eds.), John Wiley & Sons, Inc, 2010 (ISBN: 978-0-470-44467-2)

A.M. Fialho, A.M. Chakrabarty, “The role and importance of intellectual property generation and protection in drug development", Chapter 18, p. 405-419, In: Emerging Cancer Therapy: Microbial Approaches and Biotechnological tools (A.M. Fialho, A.M. Chakrabarty, Eds.), John Wiley & Sons, Inc, 2010 (ISBN: 978-0-470-44467-2)

A.M. Fialho, A.M. Chakrabarty, “Host-Pathogen Interactions: from Infection of Drug Discovery" Chapter 37, In: Text Book on Molecular Biotechnology (Basics, Applications and Modern Methods) (A. Varma, N. Verme, eds) Amity University Uttar Pradesh, India, 2009

A.M. Fialho, T.K. Das Gupta, A.M. Chakrabarty, “Promiscuous Drugs from Pathogenic Bacteria in the Post-Antibiotics Era" Capítulo 11, p. 145-162, In: Patho-Biotechnology (R. Sleator, C. Hill, eds), LANDES Bioscience, Austin, 2008

A.M. Fialho, L.M. Moreira, A.T. Granja, K. Hoffmann, A. Popescu, I. Sá-Correia, “Biotechnology of the bacterial gellan gum: genes and enzymes of the biosynthetic pathway", In: A Portrait of State-of-the-Art Research at the Technical University, (M.S. Pereira, ed.), Springer, Dordrecht, The Netherlands, pp. 233-250, 2007

Barbas, J.M. Andrade,A.M. Fialho, C.M. Arraiano, “Ribonucleases e controlo pós-transcricional da expressão génica”. In:O Mundo do RNA: Novos Desafios e Perspectivas Futuras (Cecília M. Arraiano, A.M. Fialho, eds), Lidel Edições Técnicas, Lisboa, Portugal pp. 119-139, 2007

Sá-Correia, L. Moreira,A.M. Fialho, “Engenharia Genética",In: Biotecnologia: Fundamentos e Aplicações (M. Mota e D. Lima, eds), Lidel Edições Técnicas, Lisboa, pp. 125-161, 2003

I. Sá-Correia,M. Fialho, “Electrotransformation ofSphingomonas spp”, In: Electrotransformation of Bacteria, Lab Manual (N. Eynard and J. Teissie, eds.), Springer-Verlag, Heidelberg, Germany, 2000

Patents

Issued patents:

Fialho A.M., Bernardes N., Gonçalves J., Santos A. C. “Compositions to treat HIV infections and methods therefor” Indian patent 1126/MUM/2011, March 2012

Chakrabarty A.M., Das Gupta T.K., Yamada T., Chaudhari A., Fialho A.M., Hong C.S. “Compositions and Methods for Treating HIV Infection with Cupredoxin and Cytochrome c” US Patent 7,795,410, September 14, 2010

Hong C., Yamada T., Fialho A.M., Das Gupta T. K, Chakrabarty A.M. “Transport agents for crossing the blood-brain barrier and into brain cancer cells, and methods of use thereof” US Patent 7,807,183 B2, October 5, 2010

Das Gupta Tapas; Chakrabarty Ananda; Fialho A.M. “Compositions and methods to prevent and/or treat cancer with Pa-CARD” WO2010096754 (A1) August 26, 2010

Chakrabarty A.M., Das Gupta T.K., Yamada T., Chaudhari A., Fialho A.M., Hong C.S. “Compositions and Methods for Treating Malaria with Cupredoxin and Cytochrome” US Patent 7,740,857 B2, June 22, 2010

Chakrabarty A.M., Das Gupta T.K., Yamada T., Fialho A.M. “Cupredoxin Derived Transport Agents and Methods of Use Thereof” US Patent 7,691,383 B2, April 6, 2010

Chakrabarty A.M., Das Gupta T.K., Yamada T., Chaudhari A., Fialho A.M., Hong C.S. “Compositions and Methods for Treating HIV Infection with Cupredoxin and Cytochrome c” US Patent 7,511,117 de 31 de Março, 2009

Chakrabarty A.M., Das Gupta T.K., Yamada T., Chaudhari A., Fialho A.M., Zhu Y. “Compositions and Methods for Treating Conditions Related to Ephrin Signaling with Cupredoxins” US Patent 7,381,701, June 3, 2008

Chakrabarty A.M., Das Gupta T.K., Yamada T., Chaudhari A., Fialho A.M., Hong C.S. “Compositions and Methods for Treating HIV Infection with Cupredoxin and Cytochrome c” US Patent 7,301,010 B2, November 27, 2007 

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