Research Topics

  • Epidemiology and elucidation of adaptive evolution mechanisms adopted by Burkholderia cepacia complex bacteria in respiratory infections of cystic fibrosis patients during long-term lung infection
  • New persistence and virulence factors and mechanisms that may inspire new therapeutic strategies to treat severe chronic respiratory infections
  • The Burkholderia cepacia complex bacteria as contaminants of medicines and medical devices
  • Molecular and Cellular Microbiology
  • Functional and Comparative Genomics and Biospectroscopy

Selected Publications

Hassan, A.A. Maldonado, R. F Sandra Costa dos Santos, S. C. Di Lorenzo, F., Silipo, A. C. Coutinho, Cooper, V. S., Molinaro, A. Valvano,M.A., Sá-Correia I., Structure of O-antigen and hybrid biosynthetic locus in Burkholderia cenocepacia clonal variants recovered from a cystic fibrosis patient, Frontiers in Microbiology, 8:10272017, doi: 10.3389/fmicb.2017.01027

Moreira, A.S., Mil-Homens, D.*, Sousa, S.A.*, Coutinho, C.P.*, Pinto-de-Oliveira, A., Ramos, C.G., dos Santos, S.C., Fialho, A.M., Leitão, J.H., Sá-Correia, I., Variation of Burkholderia cenocepacia virulence potential during cystic fibrosis chronic lung infection. Virulence, 21:1-15, 2016, doi: 10.1080/21505594.2016.1237334

Coutinho, C.P., Barreto, C., Pereira, L., Lito, L., Melo Cristino, J., Sá-Correia, I., Incidence of Burkholderia contaminans at a cystic fibrosis centre with an unusually high representation of Burkholderia cepacia during 15 years of epidemiological surveillance. Journal of Medical Microbiology. 64(8):927-35, 2015, doi: 10.1099/jmm.0.000094

Madeira, A., Dos Santos, S.C., Santos, P.M., Coutinho, C.P., Tyrrell, J., McClean, S., Callaghan, M., Sá-Correia, I., (2013) Proteomic profiling of Burkholderia cenocepacia clonal isolates with different virulence potential retrieved from a cystic fibrosis patient during chronic lung infection. PLoS One. 8:e83065,v 2014, doi: 10.1371/journal.pone.0083065

Coutinho, C.P., dos Santos, S.C., Madeira, A., Mira, N.P., Moreira, A.S., Sá-Correia, I., Long-term colonization of the cystic fibrosis lung by Burkholderia cepacia complex bacteria: epidemiology, clonal variation, and genome-wide expression alterations. Frontiers in Cellular and Infection Microbiology. 1: 1-11, 2011, doi: 10.3389/fcimb.2011.00012

All Papers, poster presentations and oral communications

See all

Thesis

Coutinho C.P., “Differentiation of clonal variants of the Burkholderia cepacia complex isolated during chronic respiratory infection in cystic fibrosis patients with FTIR spectroscopy". PhD Thesis, Faculty of Pharmacy, University of Porto, Porto, 2011

Papers in international journals

Hassan, A. A., Maldonado, R. F., dos Santos, S. C., Lorenzo, F. D., Silipo, A., Coutinho, C. P., Cooper, V. S., Molinaro, A., Valvano, M. A., Sá-Correia, I., Structure of O-antigen and hybrid biosynthetic locus in Burkholderia cenocepacia clonal variants recovered from a cystic fibrosis patient, Frontiers in Microbiology, 8:1027, 2017.

Guadalupe Cabral, M., Pereira, M., Silva, Z., Iria, I., Coutinho, C., Lopes, A., Sá-Correia, I., Videira, P. A., Using dendritic cells to evaluate how Burkholderia cenocepacia clonal isolates from a chronically infected cystic fibrosis patient subvert immune functions, Medical Microbiology and Immunology, 206(2), 111–123, 2017.

Moreira, A.S., Mil-Homens, D.*, Sousa, S.A.*, Coutinho, C.P.*, Pinto-de-Oliveira, A., Ramos, C.G., dos Santos, S.C., Fialho, A.M., Leitão, J.H., Sá-Correia, I., Variation of Burkholderia cenocepacia virulence potential during cystic fibrosis chronic lung infection. Virulence, 21:1-15, 2016.

Araujo, R., Caramalho, R., Coutinho, C., Sá-Correia, I. SNaPBceBcon: a practical tool for identification and genotyping of Burkholderia cepacia and Burkholderia contaminansJ Clin Microbiol. 2:483-488, 2016.

Coutinho, C.P., Barreto, C., Pereira, L., Lito, L., Melo Cristino, J., Sá-Correia, I.. Incidence of Burkholderia contaminans at a cystic fibrosis centre with an unusually high representation of Burkholderia cepacia during 15 years of epidemiological surveillance. J Med Microbiol. 64(8):927-35, 2015.

Moreira, A.S., Coutinho, C.P., Avezedo, P., Lito, L., Melo-Cristino, J., Sá-Correia, I., “Burkholderia dolosa phenotypic variation during the decline in lung function of a cystic fibrosis patient along 5.5 years of chronic colonization", Journal of Medical Microbiology, 63: 594-601, 2014.

Di Lorenzo, F., Silipo, A., Lanzetta, R., Fazio, L.L., Paciello, I., Coutinho, C.P., Sá-Correia, I., Bernardini. M., Molinaro, A., 2013, Structural elucidation and biological activity of the lipooligosaccharide of a Burkholderia dolosa isolate from a cystic fibrosis patient. ChemBioChem, 14(9): 1105-15, 2013.

Eusébio, N., Coutinho, C., Sá-Correia, I., Araújo, R., 2013, SNaPBcen: a novel and practical tool for genotyping Burkholderia cenocepacia, Journal of Clinical Microbiology, 51(8): 2646-53.

Madeira, A., dos Santos, A.C., Santos, P.M., Coutinho, C.P., Tyrrell, J., McClean, S., Callaghan, M., Sá-Correia, I., Proteomic profiling of Burkholderia cenocepacia clonal isolates with different virulence potential retrieved from a cystic fibrosis patient during chronic lung infection, PLOS ONE 8(12): e83065, 2013.

Coutinho, C.P., de Carvalho, C.C.C.R., Madeira, A., Pinto-de-Oliveira, A., Sá-Correia, I., Burkholderia cenocepacia phenotypic clonal variation during three and a half years of residence in the lungs of a cystic fibrosis patient, Infection and Immunity, 79: 2950-2960, 2011.

Coutinho, C.P., dos Santos, S.C., Madeira, A., Mira, N.P., Moreira, A.S., Sá-Correia, I., Long-term colonization of the cystic fibrosis lung by Burkholderia cepacia complex bacteria: epidemiology, clonal variation and genome-wide expression alterations. Frontiers in Cellular and Infection Microbiology, 1, 12, 2011.

Madeira, A., Santos, P.M., Coutinho, C.P., Pinto-de-Oliveira, A., Sá-Correia, I., Quantitative proteomics (2-D-DIGE) reveals molecular strategies employed by Burkholderia cenocepacia to adapt to the airways of cystic fibrosis patients under antimicrobial therapy. PROTEOMICS, 11:1313-1328, 2011.

Coutinho C.P., Sá-Correia I., Lopes J.A., 2009, Use of Fourier transform infrared spectroscopy and chemometrics to discriminate clinical isolates of bacteria of the Burkholderia cepacia complex from different species and ribopatterns, Analytical and Bioanalytical Chemistry, 394(8): 2161-71, 2009

Gouveia L., Coutinho C., Mendonça E., Batista A.P., Sousa I., Bandarra N.M., Raymundo A.. Sweet biscuits with Isochrysis galbana microalgae biomass as a functional ingredient. Journal of the Science of Food and Agriculture, 88:891-896, 2008

Poster presentations in Scientific Meetings

Coutinho C.P., Sarraguça M.C., Sarraguça J.M., Soares L.M., Miranda M., Guiomar R., Machado J., Preisner O.E., Sá-Correia I., Menezes J.C., Lopes J.A., Fourier-Transform Infrared Spectroscopy in Bacteriology, Encontro Nacional REQUIMTE, 13-14 January, Fátima, Portugal, 2011.

Madeira A., Santos P.M., Coutinho C.P., Pinto-de-Oliveira A., Sá-Correia I., Burkholderia cenocepacia adaptation to antimicrobials in cystic fibrosis: a quantitative proteomics analysis, 4th EuPA Meeting and 6th ProCura Meeting, 23-27 October, Estoril, Portugal, 2010.

Madeira, A., Mil-Homens, D., Mira, N.P., Coutinho, C.P., Pinto-de-Oliveira, A., Santos, P.M., Fialho, A.M., Sá-Correia, I., Molecular strategies employed by Burkholderia cenocepacia to adapt and persist in the airways of cystic fibrosis patients, revealed by genome-wide approaches, 2nd Scientific Meeting of Institute for Biotechnology and Bioengineering , IBB, Universidade do Minho, Braga, Portugal, 23-24 October, 2010.

Madeira A., Santos P.M., Coutinho C.P., Pinto-de-Oliveira A., Sá-Correia I., Molecular strategies employed by Burkholderia cenocepacia to adapt to the airways of cystic fibrosis patients under antimicrobial therapy, as revealed by quantitative proteomics, EMBO/FEBS Lecture Course,’Host-Microbes Interactions’ (organizers: P. COSSART and R. KOLTER, 1-9 September, Spetses, Grécia, 2010.

Coutinho C.P., Sá-Correia I. and Lopes J.A., Discrimination of Burkholderia cenocepacia complex clinical isolates using Fourier Transform Infrared Spectroscopy and chemometric approaches , MICROBIOTEC’09, 28-30 November, Vilamoura, Portugal, 2009.

Coutinho C.P., Pinto-de-Oliveira A., Lopes J.A. and Sá-Correia I., Use of Fourier Transform Infrared Spectroscopy (FTIRS) and chemometrics as a new approach to typing of clinical isolates of the Burkholderia cepacia complex, 3rd FEMS Congress of European Microbiologists (FEMS 2009), 28/06-2/07, Gothenburg, Suécia, 2009.

Lopes J.A., Preisner O.E., Coutinho C.P., Rodrigues L., Henriques J.G., Sá-Correia I., Menezes J.C., Infrared spectroscopy applications in microbiology and bioprocess monitoring, 1st Scientific Meeting of Institute for Biotechnology and Bioengineering (IBB), Universidade do Algarve, 15-16 May, 2009.

Coutinho C.P., Sá-Correia I., Lopes J.A., Discrimination of Burkholderia cenocepacia complex ribopatterns by Fourier-transform infrared spectroscopy and chemometrics, Shedding Light on Disease: Optical Diagnosis for the New Millenium (SPEC 2008), 25-29 October, São José dos Campos, São Paulo, Brasil, 2008.

Coutinho C.P., Sá-Correia I., Lopes J.A., Discrimination of Burkholderia cenocepacia complex ribopatterns by Fourier-transform infrared spectroscopy and chemometrics, FPBC’08-Forum Português Biologia Computacional, Oeiras, Portugal, Livro de Resumos pág. 54, 2008.

Coutinho C.P., Almeida A.G., Meirinhos-Soares L., Lopes J.A., Identification and Discrimination of Pharmaceutical Relevant Bacteria by Intrinsic Fluorescence, XXI Encontro Nacional da SPQ, Porto, Portugal, pág. 122, CP58, 2008.

Coutinho C.P., Preisner O., Sá-Correia I., Lopes J.A, Classification of Burkholderia cenocepacia complex isolates by Fourier Transform Infrared specroscopy and chemometrics, MICROBIOTEC’07, 30Nov-3Dec, Lisboa, Portugal, Livro de Resumos S7/P60, 2007.

Coutinho C.P., Preisner O., Sá-Correia I., Lopes J.A, Classification of Burkholderia cenocepacia complex isolates by Fourier Transform Infrared specroscopy and chemometrics, FTIR Spectroscopy in Microbiological and Medical Diagnostics, 25-26 October, Berlin, Germany, 2007.

Oral presentations in Scientific Meetings

Madeira, A., Santos, P.M., Coutinho, C.P., Pinto-de-Oliveira, A., Sá-Correia, I., Quantitative proteomics (2D-DIGE) to reveal the molecular strategies employed by Burkholderia cenocepacia to adapt to the airways of cystic fibrosis patients under antimicrobial therapy, 4th EuPA Meeting and 6th ProCura Metting – 2010 Proteomics ODYSSEY towards next decades, Estoril, Portugal, 23-27 October, 2010.

Santos C., Coutinho C., Sá-Correia I., Lima N., Intact-cell MALDITOF (ICM) mass spectrometry for rapid identification and subtyping of Burkholderia cepacia complex bacteria, 2nd Scientific Meeting of Institute for Biotechnology and Bioengineering (IBB), Universidade do Minho, Braga, Portugal, 23-24 October, 2010.

Coutinho C.P., Pinto-de-Oliveira A., Lopes J.A. and Sá-Correia I.,  Use of Fourier Transform Infrared Spectroscopy (FTIRS) and chemometrics as a new approach to typing of clinical isolates of the Burkholderia cepacia complex, 3rd FEMS Congress of European Microbiologists (FEMS 2009), 28/06-2/07, Gothenburg, Suécia, 2009.

Coutinho C.P., Mendonça E., Batista A.P., Bandarra N., Raymundo A., Gouveia L., Bolachas funcionais ricas em ácidos gordos polinsaturados ω3, por incorporação de biomassa de Isochrysis galbana, 8ºEncontro de Química dos Alimentos – Sociedade Portuguesa de Química, Beja, Portugal, 2007.

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