Cristina A. Viegas

POSITION:Assistant Professor

Leader of the research theme: Xenobiotic toxicity and herbicide bioremediation

E-mail: cristina.viegas@tecnico.ulisboa.pt

Research Topics

  • Yeast-based bioassays for the assessment of the potential toxicity of xenobiotics and environmental samples
  • Toxicogenomics in the eukaryotic model S. cerevisiae to better understand responses to stress from environmentally relevant pesticides
  • Bacteria-based bioremediation strategies for the cleanup of soils polluted with chlorinated s-triazine herbicides

Research Team:

  • Sofia Sousa

Education

  • Graduation in Chemical Engineering, IST, 1984
  • M.Sc. in Biotechnology (Biochemical Engineering), IST, 1988
  • Visiting graduate student, Unité de Biochimie Physiologique/Université Catholique de Louvain, Belgium (Prof. André Goffeau), Feb.-Mar. 1991
  • Ph.D in Biotechnology (adviser: Prof. Isabel Sá-Correia) IST, 1993
  • Post-doctoral training, as visiting post-doc scientist, at the Institute of Virology and Environmental Microbiology (with Dr. Mark Bailey; Molecular Microbial Ecology group), Oxford, UK, 1995

Positions

  • Trainee Lecturer, IST (1986-1988)
  • Lecturer, IST (1988-1993)
  • Visiting Scientist, Institute of Virology and Environmental Microbiology, Oxford, UK (Jan-Aug 1995)
  • Assistant Professor, DEQB-IST (since 1993)
  • Director of the Pedagogic Organisation Office (GOP – Gabinete Organização Pedagógica), IST (2005 – 2008)
  • Responsible for the Biological Sciences Teaching Laboratory (LCB) of the DEB-IST (2002 – 2003 and since 2004)

Selected Publications

Gil, F.N., Gonçalves, A.C., Becker, J.D., Viegas, C.A. Comparative analysis of transcriptomic responses to sub-lethal levels of six environmentally relevant pesticides in Saccharomyces cerevisiae. Ecotoxicology, 27(7):871-889, 2018. (Special Issue: Emerging Advances and Challenges in Pesticide Ecotoxicology)

Gil, F.N., Belli, G., Viegas, C.A., The Saccharomyces cerevisiae response to stress caused by the herbicidal active substance alachlor requires the iron regulon transcription factor Aft1p. Environmental Microbiology 19(2): 485-499, 2017.

Lourenço, Nídia D., Franca, R.D.G., Moreira, M. A., Gil, F.N., Viegas, C.A., Pinheiro, H.M., Comparing aerobic granular sludge and flocculent sequencing batch reactor technologies for textile wastewater treatment, Biochemical Engineering Journal, 104: 57-63, 2015.

Silva, Vera P., Moreira-Santos, M., Mateus, C., Teixeira, T., Ribeiro, R., Viegas, C.A., Evaluation of Arthrobacter aurescens strain TC1 as bioaugmentation bacterium in soils contaminated with the herbicidal substance terbuthylazine. PLoS ONE 10(12):e0144978, 2015.

Gil, Fátima N., Moreira-Santos, M., Chelinho, S., Pereira, C., Feliciano, J.R., Leitão, J.H., Sousa, J.P., Ribeiro, R., Viegas, C.A., Suitability of a Saccharomyces cerevisiae-based assay to assess the toxicity of pyrimethanil sprayed soils via surface runoff: Comparison with standard aquatic and soil toxicity assays, Science of the Total Environment 505: 161-171, 2015.

Gil, Fátima N., Becker, J.D., Viegas, C.A., Potential mechanisms underlying response to effects of the fungicide pyrimethanil from gene expression profiling in Saccharomyces cerevisiae, Journal of Agricultural and Food Chemistry, 62: 5237-5247, 2014.

All Papers, Book Chapters and Patents

See all

Book Chapters

Viegas, C. A. and Moreira, L. M, “Microbiology and disinfecting agents”, In: Ana Sofia Cardoso, Cristina M. M. Almeida, Telma C. Cordeiro, Vanessa J. Gaffney (eds), “Disinfectants: Properties, Applications and Effectiveness” (Bacteriolology Research Developments series), ISBN 978-1-63485-974-5 (eBook), ISBN 978-1-63485-958-5 (Hardcover), Nova Science Publishers Inc., NY, USA, pp. 39-76 (2017). (abstract)

A. Viegas, V. Silva, C. Mateus, S. Chelinho, M. Moreira-Santos, J. Gonçalves, V. Varela, R. Ribeiro, A. M. Fialho, J. P. Sousa, „Bioremediation strategies based on Pseudomonassp. strain ADP for worst-case scenarios of soil contamination with herbicidal formulations containing chlorinated s-triazines“,  In: A. Méndez-Vilas (ed), Industrial, medical and environmental applications of microorganisms: current status and trends, ISBN Print version: 978-90-8686-243-6, ISBN E-book: 978-90-8686-795-0, Wageningen Academic Publishers, NL, pp. 59-64 (2014). (abstract)  

Viegas, C.A., Chelinho, S., Moreira-Santos, M., Costa, C., Gil, F.N., Silva, C., Lima, D., Ribeiro, R., Sousa, J.P., Fialho, A.M., “Bioremediation of soils contaminated with atrazine and other s-triazine herbicides: current state and prospects", In: Justin A. Daniels (ed), Advances in Environmental Research. Volume 6 (ISBN 978-1-61728-163-1), Nova Science Publishers, Inc., NY, USA, pp. 1-49. (2012) (abstract)

Papers

Gil, F.N., Gonçalves, A.C., Becker, J.D., Viegas, C.A. Comparative analysis of transcriptomic responses to sub-lethal levels of six environmentally relevant pesticides in Saccharomyces cerevisiae. Ecotoxicology, 27(7):871-889, 2018. (Special Issue: Emerging Advances and Challenges in Pesticide Ecotoxicology) (abstract)

Gil, F.N., Belli, G., Viegas, C.A., The Saccharomyces cerevisiae response to stress caused by the herbicidal active substance alachlor requires the iron regulon transcription factor Aft1p. Environmental Microbiology 19(2): 485-499, 2017 (abstract)

Rodrigues, P., Silvestre, J. D., Flores-Colen, I., Viegas, C. A., de Brito, J., Kurad, R., Demertzi, M., Methodology for the assessment of the ecotoxicological potential of construction materials. Materials 10(6): 649, 2017 (doi: 10.3390/ma10060649) (abstract)

Silva, V.P., Moreira-Santos, M., Mateus, C., Teixeira, T., Ribeiro, R., Viegas, C.A. 2015. Evaluation of Arthrobacter aurescens strain TC1 as bioaugmentation bacterium in soils contaminated with the herbicidal substance terbuthylazine. PLoS ONE10(12):e0144978. (abstract)

Lourenço, N.D., Franca, R.D.G., Moreira, M.A., Gil, F.N., Viegas, C.A., Pinheiro, H.M. 2015. Comparing aerobic granular sludge and flocculent sequencing batch reactor technologies for textile wastewater treatment, Biochemical Engineering Journal, 104: 57-63. (abstract)

Gil, F.N., Moreira-Santos, M., Chelinho, S., Pereira, C., Feliciano, J.R., Leitão, J.H., Sousa, J.P., Ribeiro, R., Viegas, C.A. 2015. “Suitability of a Saccharomyces cerevisiae-based assay to assess the toxicity of pyrimethanil sprayed soils via surface runoff: Comparison with standard aquatic and soil toxicity assays”, Science of the Total Environment, 505: 161-171. (DOI: 10.1016/j.scitotenv.2014.09.094) (abstract)

Gil, F.N., Becker, J.D., Viegas, C.A. 2014. “Potential Mechanisms Underlying Response to Effects of the Fungicide Pyrimethanil from Gene Expression Profiling in Saccharomyces cerevisiaeJournal of Agricultural and Food Chemistry. Published online (DOI: 10.1021/jf5007775) (abstract)

Chelinho, S.,  Moreira-Santos, M., Silva, C.,  Costa, C., Viana, P.,  Viegas, C.A.,  Fialho, A.M., Ribeiro, R., Sousa, J.P. 2012. Semi-field testing of a bioremediation tool for atrazine contaminated soils: evaluating the efficacy on soil and aquatic compartments. Environmental Toxicology and Chemistry (doi: 10.1002/etc.1840) (abstract).

Viegas, C.A., Costa, C., André, S., Viana, P., Ribeiro, R., Moreira-Santos, M. 2012. Does S-metolachlor affect the performance of Pseudomonas sp. strain ADP as bioaugmentation bacterium for atrazine-contaminated soils? PLoS ONE 7(5): e37140, 2012 (abstract).

Gil, F.N., Gonçalves, A.C., Jacinto, M.J., Becker, J.D., Viegas, C.A., Transcriptional profiling in Saccharomyces cerevisiae relevant for predicting alachlor mechanisms of toxicity, Environmental Toxicology and Chemistry, 30, 2506-2518, 2011 (abstract)

Mendes, S., Farinha, A., Ramos, C.G., Leitão, J.H., Viegas, C.A., Martins, L.O., Synergistic action of azoreductase and laccase leads to maximal decolourization and detoxification of model dye-containing wastewaters, Bioresource Technology, 102, 9852-9859, 2011 (abstract)

Chelinho, M. Moreira-Santos, D. Lima, C. Silva, P. Viana, S. André, I Lopes, R. Ribeiro, A.M. Fialho, C.A. Viegas, J.P. Sousa. 2009. “Cleanup of atrazine contaminated soils: ecotoxicological study on the efficacy of a bioremediation tool with Pseudomonassp. ADP". Journal of Soils and Sediments, 10, 568-578, 2010 (abstract)

Pereira, A.V. Coelho, C.A. Viegas, C. Ganachaud, G. Lacazio, T. Tron, M.P. Robalo, L.O. Martins.  On the mechanism of biotransformation of the anthraquinonic dyce Acid Blue 62 by microbial laccase. Adv. Synthesis & Catalysis351, 1857-1865, 2009 (abstract)

Lima, P. Viana, S. André, S. Chelinho, C. Costa, R. Ribeiro, J.P. Sousa, A.M. Fialho, C.A. Viegas. Evaluating a bioremediation tool for atrazine contamination soies in open soil microcosmos: the effectiveness of bioaugmentation and biostimulation approaches. Chemosphere74, 187, 2009 (abstract)

Pereira, A.V. Coelho, C.A. Viegas, M.M.C. dos Santos, M.P. Robalo, L.O. Martins. Enzymatic biotransformation and detoxification of the azo dye Sudan Orange G with bacterial CotA-laccase, J. Biotechnol.139, 68-77, 2009 (abstract)

C.A. Viegas, M.G. Cabral, M.C. Teixeira, G. Neumann, H.J. Heipieper, I. Sá-Correia, Yeast adaptation to 2,4-dichlorophenoxyacetic acid involves increased membrane fatty acid saturation degree and decreased OLE1 transcription, Biochemical and Biophysical Research Communications330: 271-278, 2005 (abstract)

M.G. Cabral,  I. Sá-Correia, C.A. Viegas, Adaptative responses in yeast to the herbicide 2-methyl-4-chlorophenoxyacetic acid at the level of intracellular pH homeostasis, Journal of Applied Microbiology96, 603-612, 2004 (abstract)

Papaefthimiou, M.G. Cabral, C. Mixailidou, C.A. Viegas, I. Sá-Correia, G. Theophilidis, “Comparison of two screening bioassays, based on the frog sciatic nerve and yeast cells, for the assessment of herbicide toxicity, Environmental Toxicology and Chemistry,  23, 1211-1218, 2004  (abstract)

M.G. Cabral, C.A. Viegas, M.C. Teixeira and I. Sá-Correia, Toxicity of chlorinated phenoxyacetic acid herbicides in the experimental eukaryotic model Saccharomyces cerevisiae: role of pH and of growth phase and size of the yeast cell population, Chemosphere51, 47-54, 2003 (abstract)

Tenreiro, P.A. Nunes, C.A. Viegas, M.S. Neves,  M.C. Teixeira,  M.G. Cabral, I. Sá-Correia, AQR1gene (ORF YNL065w) encodes a plasma membrane transporter of the Major Facilitator Superfamily that confers Resistance to Short-chain Monocarboxylic Acids and Quinidine in Saccharomyces cerevisiaeBiochemical and Biophysical Research Communications, 292, 741-748, 2002 (abstract)

 M.G. Cabral, C.A. Viegas, I. Sá-Correia, Mechanisms underlying the acquisition of resistance to octanoic-acid-induced-death following exposure of Saccharomyces cerevisiae to mild stress imposed by octanoic acid or ethanol, Archives of Microbiology175, 301-307, 2001 (abstract)

Tenreiro, P.C. Rosa, C.A. Viegas and I. Sá-Correia, Expression of AZR1gene (ORF YGR224w), encoding a plasma membrane transporter of the Major Facilitator Superfamily, is required for adaptation to acetic acid and resistance to azoles in Saccharomyces cerevisiaeYeast16, 1469-1481, 2000 (abstract)

Brôco, S. Tenreiro, C.A. Viegas and I. Sá-Correia, FLR1 gene (ORF YBR008c) is required for benomyl and methotrexate resistance in Saccharomyces cerevisiaeand its benomyl-induced-expression is dependent on Pdr3 transcriptional regulator,Yeast15, 1595-1608, 1999 (abstract)

Chambel, C.A. Viegas and I. Sá-Correia, Effect of cinnamic acid on the growth and on plasma membrane H+-ATPase activity of Saccharomyces cerevisiaeInternational Journal of Food Microbiology50, 173-179, 1999 (abstract)

C.A. Viegas, P.F. Almeida, M. Cavaco and I. Sá-Correia, The H+-ATPase in the plasma membrane of Saccharomyces cerevisiae is activated during growth latency in octanoic acid-supplemented medium following the decrease in intracellular pH and cell viability, Applied and Environmental Microbiology64, 779-783, 1998 (abstract)

Carmelo, R. Santos, C.A. Viegas, I. Sá-Correia, Modification of Saccharomyces cerevisiaethermotolerance following rapid exposure to acid stress", International Journal of Food Microbiology42, 225-230, 1998  (abstract)

A. Viegas and I. Sá-Correia, Effects of low temperatures (9-33ºC) and pH (3.3-5.7) in the loss of Saccharomyces cerevisiaeviability by combining lethal concentrations of ethanol with octanoic and decanoic acids, International Journal of Food Microbiology34, 267-277, 1997 (abstract)

C.A. Viegas, A.K. Lilley, K. Bruce and M.J. Bailey, Description of a novel plasmid replicative origin from a genetically distinct family of conjugative plasmids associated with phytosphere microflora. FEMS Microbiology Letters149, 121-128, 1997 (abstract)

A. Viegas, P. B. Sebastião, A. G. Nunes and I. Sá-Correia, Activation of plasma membrane H+-ATPase and expression of PMA1and PMA2genes in Saccharomyces cerevisiae grown at supra-optimal temperatures, Applied and Environmental Microbiology61, 1904-1909, 1995 (abstract)

C.A. Viegas and I. Sá-Correia, Toxicity of Octanoic Acid in Saccharomyces cerevisiae at Temperatures between 8.5 and 30°C, Enzyme and Microbial Technology17, 826-831, 1995(abstract)

C.A. Viegas, P. Supply, E. Capieaux, L. van Dyck, A. Goffeau and I. Sá-Correia, Regulation of the expression of the H+-ATPase genes PMA1 and PMA2 during growth and effects of octanoic acid in Saccharomyces cerevisiaeBiochimica Biophysica Acta1217, 74-80, 1994 (abstract)

A. Viegas and I. Sá-Correia, Activation of Plasma Membrane ATPase of Saccharomyces cerevisiaeby octanoic acid, Journal of General Microbiology137, 645-651, 1991 (abstract)

 

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