Dalila Mil-Homens

POSITION: Post-Doc Researcher

RESEARCH THEME: Bacterial pathogenesis and therapeutic products

E-mail: dalilamil-homens@tecnico.ulisboa.pt

Research Topics

  • Adhesion events in Burkholderia cenocepacia pathogenicity associated with infection of the human respiratory tract
  • Identification and characterization of trimeric autotransporter adhesins, in the cystic fibrosis pathogen B. cenocepacia.
  • B. cenocepacia interactions with the host airway epithelial cells and immune system
  • Isolation and purification of Bcc outer membrane vesicles for vaccine production
  • Alternative antimicrobial agents against multiresistant emerging pathogens
  •  Use of Galleria mellonella wax moth larvae as an infection model host

Selected Publications

Mil-Homens D, Pinto SN, Matos RG, Arraiano C, Fialho AM. Burkholderia cenocepacia K56-2 trimeric autotransporter adhesin BcaA binds TNFR1 and contributes to induce airway inflammation. Cell Microbiol. 2017 Apr;19(4)

Moreira, A.S., Mil-Homens D.*, Sousa S.A.*, Coutinho C.P.*, Pinto-de-Oliveira A., Ramos C.G., Dos Santos S.C., Fialho A.M., Leitão J.H., Sá-Correia I., “Variation of Burkholderia cenocepacia virulence potential during cystic fibrosis chronic lung infection”. 21, 1-15, 2016. (* Contributed equally to this study)

Mil-Homens D, Ferreira-Dias S, Fialho AM. Fish oils against Burkholderia and Pseudomonas aeruginosa: in vitro efficacy and their therapeutic and prophylactic effects on infected Galleria mellonella larvae. J Appl Microbiol. 2016 Jun;120(6):1509-19.

Mil-Homens D, Leça MI, Fernandes F, Pinto SN, Fialho AM. Characterization of BCAM0224, a multifunctional trimeric autotransporter from the human pathogen Burkholderia cenocepacia. J Bacteriol. 2014 Jun;196(11):1968-79.

El-Kirat-Chatel S, Mil-Homens D, Beaussart A, Fialho AM, Dufrêne YF. Single molecule atomic force microscopy unravels the binding mechanism of a Burkholderia cenocepacia trimeric autotransporter adhesin. Mol Microbiol. 2013 Aug;89(4):649-59

All Papers and publications

See all

Mil-Homens D, Pinto SN, Matos RG, Arraiano C, Fialho AM. Burkholderia cenocepacia K56-2 trimeric autotransporter adhesin BcaA binds TNFR1 and contributes to induce airway inflammation.Cellular Microbiology, 2016. doi: 10.1111/cmi.12677.

Santos R, Costa C, Mil-Homens D, Romão D, de Carvalho CC, Pais P, Mira NP, Fialho AM, Teixeira MC. The multidrug resistance transporters CgTpo1_1 and CgTpo1_2 play a role in virulence and biofilm formation in the human pathogen Candida glabrata. Cellular Microbiology, 2016. doi: 10.1111/cmi.12686.

Moreira AS, Mil-Homens D, Sousa SA, Coutinho CP, Pinto-de-Oliveira A, Ramos CG, Dos Santos SC, Fialho AM, Leitão JH, Sá-Correia I. Variation of Burkholderia cenocepacia virulence potential during cystic fibrosis chronic lung infection. Virulence, 2016; 21:1-15. doi: 10.1080/21505594.2016.1237334.

Mil-Homens D, Ferreira-Dias S, Fialho AM. Fish oils against Burkholderia and Pseudomonas aeruginosa: in vitro efficacy and their therapeutic and prophylactic effects on infected Galleria mellonellalarvae. Journal of Applied Microbiology, 2016; 120(6):1509-19. doi: 10.1111/jam.13145.

Mil-Homens D, Leça MI, Fernandes F, Pinto SN, Fialho AM Characterization of BCAM0224, a multifunctional trimeric autotransporter from the human pathogen Burkholderia cenocepacia. Journal of Bacteriology, 2014; 196(11):1968-79. doi: 10.1128/JB.00061-14.

Viegas SC, Mil-Homens D, Fialho AM, Arraiano CM. The virulence of Salmonella enterica Serovar Typhimurium in the insect model galleria mellonella is impaired by mutations in RNase E and RNase III. Applied and Environmental Microbiology, 2013; 79(19):6124-33. doi: 10.1128/AEM.02044-13.

El-Kirat-Chatel S, Mil-Homens D, Beaussart A, Fialho AM, Dufrêne YF. Single-molecule atomic force microscopy unravels the binding mechanism of a Burkholderia cenocepacia trimeric autotransporter adhesin. Molecular Microbiology, 2013; 89(4):649-59. doi: 10.1111/mmi.12301.

Mil-Homens D, Fialho AM. A BCAM0223 mutant of Burkholderia cenocepacia is deficient in hemagglutination, serum resistance, adhesion to epithelial cells and virulence. PlosONE, 2013; 7(7):e41747. doi: 10.1371/journal.pone.0041747. 

Mil-Homens D, Bernardes N, Fialho AM. The antibacterial properties of docosahexaenoic omega-3 fatty acid against the cystic fibrosis multiresistant pathogen Burkholderia cenocepacia. FEMS Microbiology Letter, 2012; 328(1):61-9.  doi: 10.1111/j.1574-6968.2011.02476.x.

Silva IN, Ferreira AS, Becker JD, Zlosnik JE, Speert DP, He J, Mil-Homens D, Moreira LM. Mucoid morphotype variation of Burkholderia multivorans during chronic cystic fibrosis lung infection is correlated with changes in metabolism, motility, biofilm formation and virulence. Microbiology, 2011; 157(Pt 11):3124-37. doi: 10.1099/mic.0.050989-0. 

Rocha J, Popescu AO, Borges P, Mil-Homens D, Moreira LM, Sá-Correia I, Fialho AM, Frazão C Structure of Burkholderia cepacia UDP-glucose dehydrogenase (UGD) BceC and role of Tyr10 in final hydrolysis of UGD thioester intermediate. Journal of Bacteriology, 2011; 193(15):3978-87. doi: 10.1128/JB.01076-10.

Mil-Homens D, Fialho AM. Trimeric autotransporter adhesins in members of the Burkholderia cepacia complex: a multifunctional family of proteins implicated in virulence. Frontiers in Cellular and Infection Microbiology, 2011; 1:13. doi: 10.3389/fcimb.2011.00013..

Mil-Homens D, Rocha EPC, Fialho AM. Genome-wide analysis of DNA repeats in Burkholderia cenocepacia J2315 identifies a novel adhesin-like gene unique to epidemic-associated strains of the ET-12 lineage. Microbiology, 2010; 156(4): 1084-1096.  doi: 10.1099/mic.0.032623-0.

Holden MT, Seth-Smith HM, Crossman LC, Sebaihia M, Bentley SD, Cerdeño-Tárraga AM, Thomson NR, Bason N, Quail MA, Sharp S, Cherevach I, Churcher C, Goodhead I, Hauser H, Holroyd N, Mungall K, Scott P, Walker D, White B, Rose H, Iversen P, Mil-Homens D, Rocha EP, Fialho AM, Baldwin A, Dowson C, Barrell BG, Govan JR, Vandamme P, Hart CA, Mahenthiralingam E, Parkhill J. The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients. Journal of Bacteriology, 2009; 191(1):261-77. doi: 10.1128/JB.01230-08.

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